Composite

Part:BBa_K311000:Design

Designed by: Matthew Adams, Poonam Srivastava, Ian Windsor   Group: iGEM10_Minnesota   (2010-10-15)


Constitutive tet promoter with mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Plasmid Bba_J61002 was digested with EcoRI and PstI enzymes and ligated into EcoR I and Pst I cut plasmid pSB1C3.


Source

part BBa_J23105 in plasmid Bba_J61002

References